Fluid Thioglycollate Medium with Indicator and Dual-Port Septa Cap, 10mL
Details
Hardy Diagnostics K282 Fluid Thioglycollate Medium (FTM) with Indicator is a sterile, ready-to-use medium supplied as a 10 mL fill in a 16 × 125 mm glass tube for cultivating aerobic, microaerophilic, and anaerobic microorganisms during pharmaceutical sterility and environmental testing. Its red dual-port flush septa cap with distinct circle and squircle ports facilitates rapid port identification for injection, withdrawal, or venting procedures while helping reduce the potential for cross-contamination between ports. For laboratory use only; not intended for human diagnostic use.
Material/Structure
- Glass culture-media tube
- Dimensions: 16 × 125 mm
- Fill volume: 10 mL
- Red dual-port flush septa cap
- One circular port and one squircle-shaped port
- Ports support injection, withdrawal, and venting procedures
- Single-use prepared-media configuration
- Sterile
Formulation
- Fluid Thioglycollate Medium with resazurin oxidation-reduction indicator
- Pancreatic digest of casein: 15.0 g/L
- Dextrose: 5.5 g/L
- Yeast extract: 5.0 g/L
- Sodium chloride: 2.5 g/L
- Sodium thioglycollate: 0.5 g/L
- L-cystine: 0.5 g/L
- Agar: 0.75 g/L
- Resazurin indicator: 1.0 mg/L
- Final pH: 7.1 ± 0.2 at 25°C
- Translucent, light-amber appearance with a pink layer where oxygen is present
Cleanroom Environment
- Designed for sterility-testing procedures performed in pharmaceutical manufacturing and controlled laboratory environments.
- Flush septa ports allow media access without removing the cap during appropriate injection, withdrawal, and venting procedures.
- Distinct circle and squircle port shapes support rapid identification and help prevent cross-contamination between ports.
- The larger squircle port facilitates identification during telemanipulator procedures performed in isolators.
- Manufactured by Hardy Diagnostics under an ISO 13485-certified quality management system.
Benefits
- Ready-to-use prepared medium eliminates reconstitution before testing.
- Dual-port cap supports separate injection, withdrawal, or venting procedures.
- Differently shaped ports provide tactile and visual identification during controlled manipulations.
- Resazurin indicator turns pink when increased oxidation is present.
- Sodium thioglycollate maintains a low-oxygen environment that supports anaerobic microorganism recovery.
- Small agar content limits oxygen diffusion and supports the growth of small inocula and anaerobic organisms.
- 10 mL fill provides a convenient format for laboratory testing procedures.
Applications
- Pharmaceutical sterility testing
- Environmental testing
- Cultivation of aerobic microorganisms
- Cultivation of microaerophilic microorganisms
- Cultivation of anaerobic microorganisms
- Testing of normally sterile materials
- Isolator and telemanipulator procedures
- Injection, withdrawal, and venting procedures requiring separate access ports
Storage
- Store at 2–25°C.
- Keep away from direct light.
- Protect from excessive heat and freezing.
- Do not use after the labeled expiration date or if shrinking, cracking, discoloration, contamination, or other deterioration is observed.